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KMID : 0380020070220010048
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Korean Journal of Biotechnology and Bioengineering
2007 Volume.22 No. 1 p.48 ~ p.52
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The Early Detection of the Protein Toxin using Sanification and Fluorescent Dye in the Field
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Ha Yeon-Chul
Choi Ki-Bong
Kim Seong-Joo
Choi Jung-do
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Abstract
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This study was carried out to establish the optimum disruption condition of a sonificator for the protein toxin for the purpose of developing automatic biological agent detector equipped a sonificator. One of the best-known collisional quenchers is molecular oxygen, which quenches almost all known fluorophores. The sonification does an excellent job of degassing, which decreased the quenching effect and increased the fluorescence quantity. The fluorescence measurement for the protein using 0.7 X fluorescent dye concentration and above must be done in 1 minute and the fluorescence measurement for the protein using 0.3 X fluorescent dye concentration and below has to be done between 2 and 3 minute. The fluorescence quantity of the sonificatied protein sample was much higher that of the non-sonificatied protein sample. Sonificating the sample turned out to be favorable for the fluorescence measurement when measuring at the low protein concentration.
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KEYWORD
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Sonification, fluorescence, protein
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